invivo mab anti-mouse/rat il-1β blocking antibody clone b122  (Bio X Cell)

Journal: Scientific Reports

Article Title: Intense impact of IL-1β expressing inflammatory macrophages in acute aortic dissection

doi: 10.1038/s41598-024-65931-3

Figure Lengend Snippet: In mice, treatment with angiotensin II (Ang II) and β-aminopropionitrile (BAPN) causes acute inflammation before aortic dissection occurs. ( A ) Gross morphology of aortic dissection (AD) in male C57BL/6J mice; aortas in control mice without Ang II or BAPN treatment (Control), aortas without AD after exposure to ANGII (1 µg/kg/µl) and BAPN (5 g/drinking water) (No-AD), and aortas with AD after exposure to ANGII and BAPN (AD). ( B , C ) Flow cytometric analysis showing percentages with plots after gating of CD45 + immune cells excluding doublets ( B ) and cell numbers of total CD45 + cells, neutrophils, Ly6C high monocytes, and macrophages ( C ). (n = 5 controls, n = 4 Non-AD, n = 5 AD). One-way analysis of variance with Tukey’s multiple comparison test; * P < 0.05. ( D ) Uniform manifold approximation and projection (UMAP) dimensionality reduction analysis identifying unique immune populations in male mice exposed to Ang II (1 µg/kg/µl) and BAPN (5 g/l in drinking water) for 1 week. ( E ) Dot plots displaying the signature cell gene expression markers. ( F , G ) CellChat showing the activated interaction between each cell population in the interleukin-1 (IL-1) signaling pathway. ( H ) Feature plots of IL-1β and Il1r1 expression on UMAP. CellChat; a tool that can quantitatively infer and analyze intercellular communication networks from scRNA-seq data. Macs macrophages, Monos monocytes, DCs dendritic cells, NK cells natural killer cells, ILCs Innate lymphoid cells, VSMCs vascular smooth muscle cells, ECs endothelial cells.

Article Snippet: A blocking antibody against IL-1β (BE0246, BioXCell Therapeutics, New Haven, CT, USA) or an isotype control antibody (BE0091, BioXCell) were injected intraperitoneally at a dose of 200 μg every 3 days for 2 weeks (Fig. ).

Techniques: Dissection, Control, Comparison, Gene Expression, Expressing

Journal: Scientific Reports

Article Title: Intense impact of IL-1β expressing inflammatory macrophages in acute aortic dissection

doi: 10.1038/s41598-024-65931-3

Figure Lengend Snippet: IL-1β + inflammatory macrophages are accumulated before the onset of macroscopic aortic dissection (AD) in mice. ( A ) Re-clustering of monocytes and macrophages into three groups: mouse aortas without Ang II or BAPN (control), mouse aortas without AD after exposure to Ang II and BAPN (non-AD), and mouse aortas with AD after exposure to Ang II and BAPN (AD). ( B ) Violin plots depicting single-cell gene expression of each canonical monocyte or macrophage marker for clustering. ( C ) Proportion of each monocyte-macrophage cluster. ( D ) Heatmap of the top-5 differentially expressed genes in each monocyte and macrophage cluster. ( E ) Trajectory pseudo-time analysis in Monocle3 with Seurat cluster annotations (left) and change in the expression of IL-1β across pseudo-time for the monocyte and monocyte-derived macrophage partitions (clusters 0, 1, 2, 3, 4) in the non-AD and AD groups (right). Macs macrophages, Monos monocytes, Ang II Angiotensin II, BAPN β-aminopropionitrile.

Article Snippet: A blocking antibody against IL-1β (BE0246, BioXCell Therapeutics, New Haven, CT, USA) or an isotype control antibody (BE0091, BioXCell) were injected intraperitoneally at a dose of 200 μg every 3 days for 2 weeks (Fig. ).

Techniques: Dissection, Control, Gene Expression, Marker, Expressing, Derivative Assay

Journal: Scientific Reports

Article Title: Intense impact of IL-1β expressing inflammatory macrophages in acute aortic dissection

doi: 10.1038/s41598-024-65931-3

Figure Lengend Snippet: Anti-IL-1β neutralizing antibody improves survival rate in mice. ( A ) Kaplan–Meier survival curve tracking death due to ruptured aortic dissection (AD) in male C57BL/6J mice exposed to Ang II (1 µg/kg/µl) and BAPN (1 g/l in drinking water) for 2 weeks after treatment with isotype control or anti-IL-1β neutralizing antibody (isotype IgG or anti-IL-1β neutralizing antibody; 200 β g i.p./mouse/every 3 days, n = 14 sham; n = 14 anti-IL-1β neutralizing antibody). Log-rank (Mantel–Cox) test, *P < 0.05. ( B ) Incidence of AD in AD model mice treated with the isotype control or anti-IL-1β antibody (n = 11/14 sham; n = 7/14 anti-IL-1β neutralizing antibody). ( C ) Blood pressure of AD model mice treated with the isotype control or anti-IL-1β neutralizing antibody (n = 8, sham; n = 9, anti-IL-1β neutralizing antibody). ( D ) Representative images of Elastica van Gieson (EVG) staining in the ascending (upper) and thoracoabdominal aorta (bottom). ( E ) Percentage of EVG-stained area per total tunica media without AD in the ascending aorta (upper) and thoracoabdominal aorta (bottom). *P < 0.05. Ang II Angiotensin II, BAPN β-aminopropionitrile.

Article Snippet: A blocking antibody against IL-1β (BE0246, BioXCell Therapeutics, New Haven, CT, USA) or an isotype control antibody (BE0091, BioXCell) were injected intraperitoneally at a dose of 200 μg every 3 days for 2 weeks (Fig. ).

Techniques: Dissection, Control, Staining

Journal: Scientific Reports

Article Title: Intense impact of IL-1β expressing inflammatory macrophages in acute aortic dissection

doi: 10.1038/s41598-024-65931-3

Figure Lengend Snippet: Single-cell RNA sequencing reveals a characteristic immune cell landscape in the ascending aorta in patients with Stanford type A acute aortic dissection (AAD). ( A ) Representative contrast-enhanced computed tomography (CT) image of Stanford type A AAD. ( B ) Histological images stained with hematoxylin and eosin (HE) and Elastica van Gieson (EVG). ( C , D ) Uniform manifold approximation and projection (UMAP) dimensionality reduction analysis identifying a unique single-cell immune landscape in the aortas of combined ( C ) and each individual Control or AAD group ( D ) (n = 3 Controls from Li et al. ; n = 2 AAD). ( E ) Dot plots displaying signature cell gene expression markers for each immune cell cluster. ( F ) Comparison of the proportions of each cluster between controls and patients with AADs.

Article Snippet: A blocking antibody against IL-1β (BE0246, BioXCell Therapeutics, New Haven, CT, USA) or an isotype control antibody (BE0091, BioXCell) were injected intraperitoneally at a dose of 200 μg every 3 days for 2 weeks (Fig. ).

Techniques: RNA Sequencing, Dissection, Computed Tomography, Staining, Control, Gene Expression, Comparison

Journal: Scientific Reports

Article Title: Intense impact of IL-1β expressing inflammatory macrophages in acute aortic dissection

doi: 10.1038/s41598-024-65931-3

Figure Lengend Snippet: Monocytes and inflammatory macrophages expressing IL-1β are accumulated in ascending aorta in patients with Stanford type A aortic dissection (AAD). ( A ) Sub-clustering of myeloid cells by uniform manifold approximation and projection (UMAP) in Controls and AADs (n = 3 Controls from LiY et al ; n = 2 AAD). ( B ) Dot plots displaying signature cell gene expression markers for each subcluster of myeloid cells. ( C ) Featured plots displaying characteristic gene expression of IL1B, NLRP3, and CCL2 in myeloid cells. ( D ) Pie charts showing the proportion of each myeloid cell cluster in the Control and AAD groups. Percentage of partitioned monocytes and monocyte-derived macrophages (clusters 0, 1, 2, and 3). ( E ) Gene ontology (GO) terms showing enriched biological processes (BP) (left) and molecular functions (MF) of clusters 0, 1, 2, 3, and 4. ( F ) Trajectory pseudo-time analysis in Monocle3 with Seurat cluster annotations (left) and change in the expression of IL-1β across pseudo-time for monocytes and monocyte-derived macrophage partitions (clusters 0, 1, 2, and 3) in AAD samples (right). ( G ) Histological staining with EVG, CD68 and, IL-1β. The scale bar represents 100 μm. Macs macrophages, Monos monocytes, cDC conventional dendritic cells, pDCs plasmacytoid dendritic cells.

Article Snippet: A blocking antibody against IL-1β (BE0246, BioXCell Therapeutics, New Haven, CT, USA) or an isotype control antibody (BE0091, BioXCell) were injected intraperitoneally at a dose of 200 μg every 3 days for 2 weeks (Fig. ).

Techniques: Expressing, Dissection, Gene Expression, Control, Derivative Assay, Staining